|
KMID : 0613820140240030323
|
|
Journal of Life Science
2014 Volume.24 No. 3 p.323 ~ p.328
|
|
|
Transcriptional Regulation of MicroRNA-17 by PPARγ in Adipogenesis
|
|
Bae In-Seon
Kim Hyun-Ji
Chung Ki-Yong
Choi In-Ho
Kim Sang-Hoon
|
|
|
Abstract
|
|
|
|
MicroRNAs comprise a family of small noncoding RNAs that modulate physiological processes, including adipogenesis. MicroRNA-17 (miR-17) promotes adipocyte differentiation and enhances lipid accumulation. The transcriptional regulation of miR-17 during adipogenesis remains unknown. In this study, we investigated whether miR-17 is a target of peroxisome proliferator-activated receptor PPARr (PPARr), which is a key regulator of adipogenesis. The levels of miR-17 and the expression of PPARr increased after the induction of adipocyte differentiation. Three putative peroxisome proliferator response elements (PPREs) were identified in the miR-17 promoter region. Using chromatin immunoprecipitation and luciferase reporter assays, we observed the interaction of PPARr with the miR-17 promoter. Mutagenesis experiments showed that the -677/-655 region of the miR-17 promoter could function as a PPRE site. These results suggest that PPARr is essential for transcriptional activation of the miR-17 gene, thereby contributing to understanding the molecular mechanism of adipogenesis in adipocytes.
|
|
|
KEYWORD
|
|
|
Adipogenesis, microRNA, peroxisome proliferator-activated receptor r, 3T3-L1 cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|